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Briefly, proteins were precipitated using methanol (1:1 v/v), and the supernatants were assessed by LC-MS/MS using a QTRAP 5500 (SCIEZ, Concord, CA, USA) and Agilent 1290 HPLC (Agilent, Santa Clara, USA) with a calibration curve (0.001–200 ng) of known amount of the molecules of interest