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Octylthioglucoside is a nonionic detergent that has been used to isolate membrane proteins without inactivation
high throughput sequencing and various contamination assays
but does not oxidize well with Gaussia (GLuc)
Substrates for HRP are luminol and benzidine substrates
Eurodenaturant: 1l of isopropanol (IPA)
Coelenterazine 400A 64742-49-0 Octylthioglucoside is a nonionic detergentCoelenterazine 400A is a derivative of coelenterazine and is a good substrate for Renilla reniformis luciferase (RLuc), but does not oxidize well with Gaussia (GLuc). It is the preferred substrate for BRET (Bioluminescence Resonance Energy Transfer) since its emission maximum of 400nm has minimal interference with the GFP emission.
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